Effect of Eucommia ulmoides Oliv., Gynostemma pentaphyllum (Thunb.) Makino, and Curcuma longa L. on Th1-and Th2-cytokine responses and human leukocyte antigen-DR expression in peripheral blood mononuclear cells of septic patients
作者 Wu, Huang-Pin; Lin, Yin-Ku
期刊 JOURNAL OF ETHNOPHARMACOLOGY , FEB 1994,2:36
摘要
Ethnopharmacological relevance: Many traditional Chinese medicines (TCM), such as Eucorrunia ulmoides Oliv., Gynostenuna pentvphyllum (Thunb.) Makino, and Curcuma longa L, have been reported to have various immune modulatory effects.Aim of the study: To determine the effects of extracts from these three TCM on type 1 T help (Th1- and Th2-cytokine responses and human leukocyte antigen (HLA)-DR expression in peripheral blood mononuclear cells (PBMCs) obtained from septic patients.Materials and methods: Lipopolysaccharide (LPS)-stimulated PBMCs of healthy controls and septic patients were cultured for 48 hs with or without 0.05/0.1 mg/ml of TCM extract. HLA-DR expression in monocytes was detected using flow cytofluorimetry. The interferon [IFN]-gamma, tumor necrosis factor [TNF]-alpha, interleukin (IL) - 2, IL-5, IL-10, and IL-13 levels in supernatants were measured with a human enzyme-linked immunosorbent assay.Results: Treatment with either 0.05 or 0.1 mg/ml of C. longa L extract significantly restored the percentage of HLA-DR-positive monocytes, which was decreased by LPS in control and patient groups. Treatment with 0.05 or 0.1 mg/nil E. ulmoides Oliv. and C. longa L. extract decreased IL-10 production from LPS-stimulated PBMCs of controls and patients. In patients with sepsis, C. Tonga L. extract decreased IL-10 production to a greater degree than did E. ulmoides Oliv extract. Although IFN-gamma, TNF-alpha, or IL-13 productions from LPS-stimulated PBMCs were influenced by E. ulmoides Oliv., G. pentaphyllunt (Thunb.) Makino, or C. longa L in control or sepsis groups in this study, only the influence of IL-10 was consistent in both control and sepsis groups.Conclusions: By enhancing monocyte HLA-DR expression and decreasing IL-10 production, C. longa L might help restore inflammatory responses in septic patients to eradicate pathogens.